PDBe 5cy1

X-ray diffraction
3.4Å resolution

Tn3 resolvase - site III complex crystal form I

Released:
Source organism: Escherichia coli
Entry authors: Montano SP, Rice PA

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transposon Tn3 resolvase Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 21.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ADI2 (Residues: 1-185; Coverage: 100%)
Gene name: tnpR
Sequence domains:
DNA (30-MER) Chain: C
Molecule details ›
Chain: C
Length: 30 nucleotides
Theoretical weight: 9.23 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (30-MER) Chain: D
Molecule details ›
Chain: D
Length: 30 nucleotides
Theoretical weight: 9.21 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 77.313Å b: 77.313Å c: 272.277Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.248 0.292
Expression systems:
  • Escherichia coli
  • Not provided