5cqx

X-ray diffraction
1.63Å resolution

E. coli MazF mutant E24A in complex with MazE residues 68-82 form I

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Endoribonuclease toxin MazF Chains: A, B
Molecule details ›
Chains: A, B
Length: 119 amino acids
Theoretical weight: 13.08 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE70 (Residues: 1-111; Coverage: 100%)
Gene names: JW2753, b2782, chpA, chpAK, mazF
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system
Structure domains: SH3 type barrels.
Antitoxin MazE Chain: C
Molecule details ›
Chain: C
Length: 15 amino acids
Theoretical weight: 1.89 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
UniProt:
  • Canonical: P0AE72 (Residues: 68-82; Coverage: 18%)
Gene names: JW2754, b2783, chpAI, chpR, mazE

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: C2
Unit cell:
a: 68.76Å b: 32.25Å c: 86.26Å
α: 90° β: 97.66° γ: 90°
R-values:
R R work R free
0.178 0.174 0.235
Expression systems:
  • Escherichia coli
  • Not provided