5ciz

X-ray diffraction
5.01Å resolution

E. coli RNA polymerase alpha subunit CTD in complex with CAP and DNA: A(5)-tract binding site for alpha CTD

Released:
Source organism: Escherichia coli K-12
Entry authors: Napoli AA, Lawson CL

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
cAMP-activated global transcriptional regulator CRP Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.54 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACJ8 (Residues: 2-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
DNA-directed RNA polymerase subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 84 amino acids
Theoretical weight: 9.36 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A7Z4 (Residues: 246-329; Coverage: 26%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
DNA (5'-D(*CP*TP*TP*TP*TP*TP*GP*CP*CP*TP*AP*AP*AP*AP*TP*GP*TP*GP*AP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
DNA (5'-D(*CP*TP*AP*GP*AP*TP*CP*AP*CP*AP*TP*TP*TP*TP*AP*GP*GP*CP*AP*AP*AP*AP*AP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 24 nucleotides
Theoretical weight: 7.39 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P6222
Unit cell:
a: 176.312Å b: 176.312Å c: 158.493Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.178 0.214
Expression system: Escherichia coli BL21(DE3)