5c56

X-ray diffraction
2.68Å resolution

Crystal structure of USP7/HAUSP in complex with ICP0

Released:
Primary publication:
Molecular mechanism for the substrate recognition of USP7.
OpenAccess logo Protein Cell 6 849-52 (2015)
PMID: 26210801

Function and Biology Details

Reactions catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase ICP0 Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.23 KDa
Source organism: Human alphaherpesvirus 1
Expression system: Not provided
UniProt:
  • Canonical: P08393 (Residues: 613-633; Coverage: 3%)
Gene names: ICP0, IE110
Ubiquitin carboxyl-terminal hydrolase 7 Chain: A
Molecule details ›
Chain: A
Length: 548 amino acids
Theoretical weight: 64.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93009 (Residues: 560-1102; Coverage: 49%)
Gene names: HAUSP, USP7
Sequence domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 78.879Å b: 80.378Å c: 151.149Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.263
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)