PDBe 5c31

X-ray diffraction
3.1Å resolution

Constitutively active Sin recombinase catalytic domain reveals two rotational intermediates

Released:
Source organism: Staphylococcus aureus
Primary publication:
Snapshots of a molecular swivel in action
Nucleic Acids Res. (2018)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative transposon Tn552 DNA-invertase bin3 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 128 amino acids
Theoretical weight: 15.15 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P20384 (Residues: 1-128; Coverage: 63%)
Gene name: bin3
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 65.589Å b: 152.719Å c: 193.753Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.227 0.286
Expression system: Escherichia coli