5btv

X-ray diffraction
1.7Å resolution

Crystal structure of human 14-3-3 sigma in complex with a Tau-protein peptide surrounding pS324

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P31947 (Residues: 1-231; Coverage: 93%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Microtubule-associated protein tau Chain: P
Molecule details ›
Chain: P
Length: 4 amino acids
Theoretical weight: 412 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10636 (Residues: 640-643; Coverage: 1%)
Gene names: MAPT, MAPTL, MTBT1, TAU

Ligands and Environments

3 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2221
Unit cell:
a: 82.55Å b: 112.31Å c: 62.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.202
Expression systems:
  • Escherichia coli
  • Not provided