X-ray diffraction
2.1Å resolution

Crystal structure of the IstB AAA+ domain bound to ADP-BeF3


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Insertion sequence IS5376 putative ATP-binding protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 189 amino acids
Theoretical weight: 21.97 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
  • Canonical: Q45619 (Residues: 63-248; Coverage: 74%)
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P61
Unit cell:
a: 133.806Å b: 133.806Å c: 70.95Å
α: 90° β: 90° γ: 120°
R R work R free
0.183 0.182 0.203
Expression system: Escherichia coli