› 5bq5

X-ray diffraction
2.1Å resolution

Crystal structure of the IstB AAA+ domain bound to ADP-BeF3

Released:
DOI: 10.2210/pdb5bq5/pdb
PDB entry 5bq5 coloured by chain, front view.
PDB entry 5bq5 coloured by chain, side view.
PDB entry 5bq5 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
An Atypical AAA+ ATPase Assembly Controls Efficient Transposition through DNA Remodeling and Transposase Recruitment.
Arias-Palomo E, Berger JM
Cell 162 860-71 (2015)
PMID: 26276634

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175067 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insertion sequence IS5376 putative ATP-binding protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 189 amino acids
Theoretical weight: 21.97 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q45619 (Residues: 63-248; Coverage: 74%)
Sequence domains: IstB-like ATP binding protein
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ADP 3 x ADP
Ligand MG 3 x MG
Ligand BEF 3 x BEF
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P61
Unit cell:
a: 133.806Å b: 133.806Å c: 70.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.182 0.203
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Review: The lord of the rings: Structure and mechanism of the sliding clamp loader.
Kelch BA. (2016)
2 more

3 mentions without citation

Structural basis of protein translocation by the Vps4-Vta1 AAA ATPase.
Monroe et al. (2017)
2 more