5b7i

X-ray diffraction
2.6Å resolution

Cas3-AcrF3 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CRISPR-associated nuclease/helicase Cas3 subtype I-F/YPEST Chain: A
Molecule details ›
Chain: A
Length: 1082 amino acids
Theoretical weight: 122.55 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02ML8 (Residues: 1-1076; Coverage: 100%)
Gene names: PA14_33340, cas3
Sequence domains: HD domain
Uncharacterized protein AcrF3 Chains: B, C
Molecule details ›
Chains: B, C
Length: 153 amino acids
Theoretical weight: 17.61 KDa
Source organism: Pseudomonas phage JBD5
Expression system: Escherichia coli
UniProt:
  • Canonical: L7P7R7 (Residues: 1-139; Coverage: 100%)
Gene name: JBD5_035

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2221
Unit cell:
a: 97.304Å b: 127.197Å c: 218.455Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.249
Expression system: Escherichia coli