5aji

X-ray diffraction
2.99Å resolution

MscS D67R1 high resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small-conductance mechanosensitive channel Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 286 amino acids
Theoretical weight: 31.1 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C0S1 (Residues: 1-286; Coverage: 100%)
Gene names: JW2891, b2924, mscS, yggB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 126.33Å b: 149.09Å c: 173.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.244 0.263
Expression system: Escherichia coli