› 5afp

X-ray diffraction
2.3Å resolution

Neuronal calcium sensor-1 (NCS-1)from Rattus norvegicus complex with rhodopsin kinase peptide from Homo sapiens

Released:
DOI: 10.2210/pdb5afp/pdb
PDB entry 5afp coloured by chain, front view.
PDB entry 5afp coloured by chain, side view.
PDB entry 5afp coloured by chain, top view.
Source organisms:
Primary publication:
Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different Modes of Interactions.
Pandalaneni S, Karuppiah V, Saleem M, Haynes LP, Burgoyne RD, Mayans O, Derrick JP, Lian LY
J Biol Chem 290 18744-56 (2015)
PMID: 25979333

Function and Biology Details

Reaction catalysed: 
ATP + [rhodopsin] = ADP + [rhodopsin] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158587 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neuronal calcium sensor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.9 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62168 (Residues: 1-190; Coverage: 100%)
Gene names: Flup, Freq, Ncs1
Sequence domains: EF hand
Structure domains: EF-hand
Rhodopsin kinase GRK1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 25 amino acids
Theoretical weight: 2.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15835 (Residues: 1-25; Coverage: 4%)
Gene names: GRK1, RHOK

Ligands and Environments

2 bound ligands:
Ligand CA 6 x CA
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 40.69Å b: 93.69Å c: 55.71Å
α: 90° β: 92.25° γ: 90°
R-values:
R R work R free
0.227 0.226 0.255
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Defining potential roles of Pb(2+) in neurotoxicity from a calciomics approach.
Gorkhali et al. (2016)
3 more

5 mentions without citation

Calcium Sensors in Neuronal Function and Dysfunction.
Burgoyne et al. (2019)
4 more