5a6c

X-ray diffraction
2.9Å resolution

Concomitant binding of Afadin to LGN and F-actin directs planar spindle orientation

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Afadin; G-protein-signaling modulator 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 41.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55196 (Residues: 1709-1746; Coverage: 2%)
  • Canonical: P81274 (Residues: 22-357; Coverage: 49%)
Gene names: AF6, AFDN, GPSM2, LGN, MLLT4
Sequence domains:
Structure domains: Tetratricopeptide repeat domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P213
Unit cell:
a: 169.88Å b: 169.88Å c: 169.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.252
Expression system: Escherichia coli BL21(DE3)