5a5t

Electron Microscopy
6Å resolution

Structure of mammalian eIF3 in the context of the 43S preinitiation complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Eukaryotic translation initiation factor 3 subunit A Chain: A
Molecule details ›
Chain: A
Length: 1362 amino acids
Theoretical weight: 164.9 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SMZ5 (Residues: 1-1362; Coverage: 100%)
Gene names: EIF3A, EIF3S10
Sequence domains: PCI domain
Eukaryotic translation initiation factor 3 subunit C Chain: C
Molecule details ›
Chain: C
Length: 843 amino acids
Theoretical weight: 97.92 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U971 (Residues: 71-913; Coverage: 92%)
Gene names: EIF3C, EIF3S8
Sequence domains: PCI domain
Eukaryotic translation initiation factor 3 subunit E Chain: E
Molecule details ›
Chain: E
Length: 445 amino acids
Theoretical weight: 52.28 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SUC8 (Residues: 18-462; Coverage: 96%)
Gene names: EIF3E, EIF3S6, INT6
Sequence domains:
EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F Chain: F
Molecule details ›
Chain: F
Length: 364 amino acids
Theoretical weight: 37.85 KDa
Source organism: Oryctolagus cuniculus
Eukaryotic translation initiation factor 3 subunit H Chain: H
Molecule details ›
Chain: H
Length: 352 amino acids
Theoretical weight: 39.95 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1ST95 (Residues: 12-363; Coverage: 97%)
Gene names: EIF3H, EIF3S3
Sequence domains:
Eukaryotic translation initiation factor 3 subunit K Chain: K
Molecule details ›
Chain: K
Length: 218 amino acids
Theoretical weight: 25.13 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T3L2 (Residues: 1-218; Coverage: 100%)
Gene names: EIF3K, EIF3S12
Sequence domains: CSN8/PSMD8/EIF3K family
Eukaryotic translation initiation factor 3 subunit L Chain: L
Molecule details ›
Chain: L
Length: 564 amino acids
Theoretical weight: 66.8 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SED9 (Residues: 44-607; Coverage: 93%)
Gene names: EIF3EIP, EIF3L, EIF3S6IP
Sequence domains: RNA polymerase I-associated factor PAF67
Eukaryotic translation initiation factor 3 subunit M Chain: M
Molecule details ›
Chain: M
Length: 374 amino acids
Theoretical weight: 42.56 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SLW8 (Residues: 1-374; Coverage: 100%)
Gene name: EIF3M
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-3056