› 4zze

X-ray diffraction
1.76Å resolution

Raffinose and panose binding protein from Bifidobacterium animalis subsp. lactis Bl-04, bound with panose

Released:
DOI: 10.2210/pdb4zze/pdb
PDB 4zze coloured by chain and viewed from the front.
PDB 4zze coloured by chain and viewed from the side.
PDB 4zze coloured by chain and viewed from the top.
Source organism: Bifidobacterium animalis subsp. lactis Bl-04
Primary publication:
An ATP Binding Cassette Transporter Mediates the Uptake of α-(1,6)-Linked Dietary Oligosaccharides in Bifidobacterium and Correlates with Competitive Growth on These Substrates.
Ejby M, Fredslund F, Andersen JM, Vujičić Žagar A, Henriksen JR, Andersen TL, Svensson B, Slotboom DJ, Abou Hachem M
J. Biol. Chem. 291 20220-31 (2016)
PMID: 27502277

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sugar binding protein of ABC transporter system Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 44.21 KDa
Source organism: Bifidobacterium animalis subsp. lactis Bl-04
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1A9TAB8 (Residues: 5-396; Coverage: 99%)
Gene name: Balac_1599
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Ligand GLC 6 x GLC
2 bound ligands:
Ligand MG 9 x MG
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 54.88Å b: 91.33Å c: 142.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.205
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Beneficial Effect of Intestinal Fermentation of Natural Polysaccharides.
Zhang et al. (2018)
2 more