› 4zza

X-ray diffraction
2.02Å resolution

Raffinose and panose binding protein from Bifidobacterium animalis subsp. lactis Bl-04, bound with raffinose, selenomethionine derivative

Released:
DOI: 10.2210/pdb4zza/pdb
PDB 4zza coloured by chain and viewed from the front.
PDB 4zza coloured by chain and viewed from the side.
PDB 4zza coloured by chain and viewed from the top.
Source organism: Bifidobacterium animalis subsp. lactis Bl-04
Primary publication:
An ATP Binding Cassette Transporter Mediates the Uptake of α-(1,6)-Linked Dietary Oligosaccharides in Bifidobacterium and Correlates with Competitive Growth on These Substrates.
Ejby M, Fredslund F, Andersen JM, Vujičić Žagar A, Henriksen JR, Andersen TL, Svensson B, Slotboom DJ, Abou Hachem M
J. Biol. Chem. 291 20220-31 (2016)
PMID: 27502277

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Sugar binding protein of ABC transporter system Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 44.68 KDa
Source organism: Bifidobacterium animalis subsp. lactis Bl-04
Expression system: Escherichia coli B
UniProt:
  • Canonical: A0A1A9TAB8 (Residues: 1-396; Coverage: 100%)
Gene name: Balac_1599
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: FRU, GLC, GLA
Ligand FRU 2 x FRU
Ligand GLC 2 x GLC
Ligand GLA 2 x GLA
No bound ligands
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P212121
Unit cell:
a: 55.385Å b: 90.949Å c: 145.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.168 0.21
Expression system: Escherichia coli B

Quick links

Citations

3 review citations

Beneficial Effect of Intestinal Fermentation of Natural Polysaccharides.
Zhang et al. (2018)
2 more