› 4zz7

X-ray diffraction
2.9Å resolution

Crystal structure of methylmalonate-semialdehyde dehydrogenase (DddC) from Oceanimonas doudoroffii

Released:
DOI: 10.2210/pdb4zz7/pdb
PDB 4zz7 coloured by chain and viewed from the front.
PDB 4zz7 coloured by chain and viewed from the side.
PDB 4zz7 coloured by chain and viewed from the top.
Source organism: Oceanimonas doudoroffii
Primary publication:
Crystal structure and modeling of the tetrahedral intermediate state of methylmalonate-semialdehyde dehydrogenase (MMSDH) from Oceanimonas doudoroffii.
Do H, Lee CW, Lee SG, Kang H, Park CM, Kim HJ, Park H, Park H, Lee JH
J. Microbiol. 54 114-21 (2016)
PMID: 26832667

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldedh domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 501 amino acids
Theoretical weight: 54.37 KDa
Source organism: Oceanimonas doudoroffii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G5CZI2 (Residues: 1-498; Coverage: 100%)
Gene names: B6S08_06410, dddC, mmsA
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NAD 6 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21212
Unit cell:
a: 156.7Å b: 160.3Å c: 238.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.246
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

Expression and Interaction Analysis among Saffron ALDHs and Crocetin Dialdehyde.
Gómez-Gómez et al. (2018)
1 more