X-ray diffraction
2.6Å resolution

X-ray crystal structure of PfA-M17 in complex with hydroxamic acid-based inhibitor 10s


Function and Biology Details

Reactions catalysed:
Release of N-terminal proline from a peptide.
Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Cytosol aminopeptidase domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 522 amino acids
Theoretical weight: 57.98 KDa
Source organism: Plasmodium falciparum FcB1/Columbia
Expression system: Escherichia coli BL21
  • Canonical: A0A024V0B1 (Residues: 84-605; Coverage: 86%)
Gene name: PFFVO_05086
Sequence domains: Cytosol aminopeptidase family, catalytic domain
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 174.436Å b: 176.565Å c: 230.997Å
α: 90° β: 90° γ: 90°
R R work R free
0.194 0.192 0.244
Expression system: Escherichia coli BL21