› 4zvy

X-ray diffraction
1.9Å resolution

Structure of human ALDH7A1 complexed with NAD+ in space group P4212

Released:
DOI: 10.2210/pdb4zvy/pdb
PDB 4zvy coloured by chain and viewed from the front.
PDB 4zvy coloured by chain and viewed from the side.
PDB 4zvy coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural Basis of Substrate Recognition by Aldehyde Dehydrogenase 7A1.
Luo M, Tanner JJ
Biochemistry (2015)
PMID: 26260980

Function and Biology Details

Reactions catalysed: 
(1a) (S)-2-amino-6-oxohexanoate = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H(2)O (spontaneous)
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Betaine aldehyde + NAD(+) + H(2)O = betaine + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-aminoadipic semialdehyde dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 513 amino acids
Theoretical weight: 55.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49419 (Residues: 29-539; Coverage: 95%)
Gene names: ALDH7A1, ATQ1
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P4212
Unit cell:
a: 159.111Å b: 159.111Å c: 79.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.213
Expression system: Escherichia coli

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Citations

3 review citations

Impact of missense mutations in the ALDH7A1 gene on enzyme structure and catalytic function.
Korasick et al. (2021)
2 more

1 mention without citation

Structural Basis of Substrate Recognition by Aldehyde Dehydrogenase 7A1.
Luo et al. (2015)