› 4zsb

X-ray diffraction
2Å resolution

Crystal structure of the ligand-free effector-binding domain of DasR (DasR-EBD)

Released:
DOI: 10.2210/pdb4zsb/pdb
PDB entry 4zsb coloured by chain, front view.
PDB entry 4zsb coloured by chain, side view.
PDB entry 4zsb coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
Crystal Structures of the Global Regulator DasR from Streptomyces coelicolor: Implications for the Allosteric Regulation of GntR/HutC Repressors.
Fillenberg SB, Friess MD, Körner S, Böckmann RA, Muller YA
PLoS One 11 e0157691 (2016)
PMID: 27337024

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional repressor DasR Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 19.03 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K492 (Residues: 89-254; Coverage: 65%)
Gene names: SC7E4.28c, SCO5231, dasR
Sequence domains: UTRA domain
Structure domains: Chorismate lyase-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3121
Unit cell:
a: 56.634Å b: 56.634Å c: 108.995Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.212 0.256
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era.
van der Heul et al. (2018)
4 more

1 mention without citation

Crystal Structures of the Global Regulator DasR from Streptomyces coelicolor: Implications for the Allosteric Regulation of GntR/HutC Repressors.
Fillenberg et al. (2016)