PDBe 4zrt

X-ray diffraction
1.74Å resolution

PTP1BC215S bound to Nephrin peptide substrate

Released:

Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 34.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-298; Coverage: 69%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
Nephrin Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.39 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O60500 (Residues: 1188-1198; Coverage: 1%)
Gene names: NPHN, NPHS1

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 43.848Å b: 88.725Å c: 50.043Å
α: 90° β: 97.25° γ: 90°
R-values:
R R work R free
0.17 0.168 0.207
Expression systems:
  • Escherichia coli
  • Not provided