4zph

X-ray diffraction
2.8Å resolution

Crystal Structure of the Heterodimeric HIF-2a:ARNT Complex with Proflavine

Released:
Source organism: Mus musculus
Primary publication:
Structural integration in hypoxia-inducible factors.
Nature (2015)
PMID: 26245371

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aryl hydrocarbon receptor nuclear translocator Chains: A, C
Molecule details ›
Chains: A, C
Length: 384 amino acids
Theoretical weight: 43.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P53762 (Residues: 81-464; Coverage: 49%)
Gene name: Arnt
Sequence domains:
Endothelial PAS domain-containing protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 360 amino acids
Theoretical weight: 41.2 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P97481 (Residues: 3-361; Coverage: 41%)
Gene names: Epas1, Hif2a
Sequence domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 48.896Å b: 76.418Å c: 98.345Å
α: 89.83° β: 89.64° γ: 73.21°
R-values:
R R work R free
0.189 0.185 0.221
Expression system: Escherichia coli BL21