4zpd

X-ray diffraction
1.8Å resolution

Coxsackievirus B3 Polymerase - A345V mutant

Released:

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 462 amino acids
Theoretical weight: 52.51 KDa
Source organism: Coxsackievirus B3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5UEA2 (Residues: 1724-2185; Coverage: 21%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P43212
Unit cell:
a: 74.45Å b: 74.45Å c: 289.638Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.229
Expression system: Escherichia coli BL21(DE3)