› 4zpd

X-ray diffraction
1.8Å resolution

Coxsackievirus B3 Polymerase - A345V mutant

Released:
DOI: 10.2210/pdb4zpd/pdb
PDB 4zpd coloured by chain and viewed from the front.
PDB 4zpd coloured by chain and viewed from the side.
PDB 4zpd coloured by chain and viewed from the top.
Source organism: Coxsackievirus B3
Primary publication:
Design of a Genetically Stable High Fidelity Coxsackievirus B3 Polymerase That Attenuates Virus Growth in Vivo.
McDonald S, Block A, Beaucourt S, Moratorio G, Vignuzzi M, Peersen OB
J Biol Chem 291 13999-14011 (2016)
PMID: 27137934

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 462 amino acids
Theoretical weight: 52.51 KDa
Source organism: Coxsackievirus B3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5UEA2 (Residues: 1724-2185; Coverage: 21%)
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P43212
Unit cell:
a: 74.45Å b: 74.45Å c: 289.638Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Evolutionary and Structural Overview of Human Picornavirus Capsid Antibody Evasion.
Cifuente et al. (2019)
8 more