› 4zp2

X-ray diffraction
2.2Å resolution

Crystal structure of E. coli multidrug transporter MdfA in complex with n-dodecyl-N,N-dimethylamine-N-oxide

Released:
DOI: 10.2210/pdb4zp2/pdb
PDB 4zp2 coloured by chain and viewed from the front.
PDB 4zp2 coloured by chain and viewed from the side.
PDB 4zp2 coloured by chain and viewed from the top.
Source organism: Escherichia coli K-12
Primary publication:
Substrate-bound structure of the E. coli multidrug resistance transporter MdfA.
Heng J, Zhao Y, Liu M, Liu Y, Fan J, Wang X, Zhao Y, Zhang XC
Cell Res. (2015)
PMID: 26238402

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug transporter MdfA Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 42.46 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0AEY8 (Residues: 9-400; Coverage: 96%)
Gene names: JW0826, b0842, cmlA, cmr, mdfA
Sequence domains: Major Facilitator Superfamily

Ligands and Environments

1 bound ligand:
Ligand LDA 2 x LDA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 94.086Å b: 64.085Å c: 101.253Å
α: 90° β: 104.19° γ: 90°
R-values:
R R work R free
0.208 0.206 0.232
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

15 review citations

Membrane Efflux Pumps of Pathogenic Vibrio Species: Role in Antimicrobial Resistance and Virulence.
Stephen et al. (2022)
14 more

9 mentions without citation

Drug Transporters in the Kidney: Perspectives on Species Differences, Disease Status, and Molecular Docking.
Zou et al. (2021)
8 more