4zp2

X-ray diffraction
2.2Å resolution

Crystal structure of E. coli multidrug transporter MdfA in complex with n-dodecyl-N,N-dimethylamine-N-oxide

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multidrug transporter MdfA Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 42.46 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0AEY8 (Residues: 9-400; Coverage: 96%)
Gene names: JW0826, b0842, cmlA, cmr, mdfA
Sequence domains: Major Facilitator Superfamily

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 94.086Å b: 64.085Å c: 101.253Å
α: 90° β: 104.19° γ: 90°
R-values:
R R work R free
0.208 0.206 0.232
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'