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Crystal structure of the Chaetomium thermophilum Sqt1 bound to the N-terminus of the ribosomal protein L10

X-ray diffraction
1.7Å resolution
PDB entry 4zoz coloured by chain, front view.
PDB entry 4zoz coloured by chain, side view.
PDB entry 4zoz coloured by chain, top view.
Source organism: Thermochaetoides thermophila
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: 60S ribosomal protein L10-like protein and sqt1
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Multimeric state: hetero dimer
Accessible surface area: 15707.5 Å2
Buried surface area: 1369.74 Å2
Dissociation area: 684.87 Å2
Dissociation energy (ΔGdiss): 3.2 kcal/mol
Dissociation entropy (TΔSdiss): 7.92 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-123947
Assembly 2
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Assembly Name: 60S ribosomal protein L10-like protein and sqt1
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Multimeric state: hetero dimer
Accessible surface area: 15598.42 Å2
Buried surface area: 1401.33 Å2
Dissociation area: 700.67 Å2
Dissociation energy (ΔGdiss): 2.85 kcal/mol
Dissociation entropy (TΔSdiss): 7.91 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-123947

Macromolecules

Sqt1
Chains: A, B
Length: 480 amino acids
Theoretical weight: 50.3 KDa
Source organism: Thermochaetoides thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0S0R0 (Residues: 54-533; Coverage: 90%)
Gene name: CTHT_0010920
Pfam: WD domain, G-beta repeat
InterPro:
CATH: YVTN repeat-like/Quinoprotein amine dehydrogenase
Sqt1 in PDB entry 4zoz, assembly 1, front view.
Sqt1 in PDB entry 4zoz, assembly 1, side view.
Sqt1 in PDB entry 4zoz, assembly 1, top view.
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60S ribosomal protein L10-like protein
Chains: C, Y
Length: 28 amino acids
Theoretical weight: 3.47 KDa
Source organism: Thermochaetoides thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0SEI1 (Residues: 1-20; Coverage: 9%)
Gene name: CTHT_0063830
InterPro: Large ribosomal subunit protein uL16 superfamily
60S ribosomal protein L10-like protein in PDB entry 4zoz, assembly 1, front view.
60S ribosomal protein L10-like protein in PDB entry 4zoz, assembly 1, side view.
60S ribosomal protein L10-like protein in PDB entry 4zoz, assembly 1, top view.
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