4zny

X-ray diffraction
2.4Å resolution

Structure of the human TSG101-UEV Domain in complex with the PTAP motif of the p19 gag protein of the Human T-cell Leukemia type I virus

Released:
Entry authors: Camara-Artigas A, Bacarizo J

Function and Biology Details

Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor susceptibility gene 101 protein Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 16.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99816 (Residues: 4-145; Coverage: 36%)
Gene name: TSG101
Sequence domains: UEV domain
Structure domains: Ubiquitin Conjugating Enzyme
Matrix protein p19 Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.12 KDa
Source organism: Human T-cell leukemia virus type I
Expression system: Not provided
UniProt:
  • Canonical: P14078 (Residues: 121-130; Coverage: 1%)
Gene name: gag-pro-pol

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 78.622Å b: 119.743Å c: 41.641Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.23 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided