4zn0

X-ray diffraction
2.6Å resolution

Structure of the NADPH-dependent thioredoxin reductase from Methanosarcina mazei

Released:

Function and Biology Details

Reaction catalysed:
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyr_redox_2 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 311 amino acids
Theoretical weight: 33.89 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8PUI1 (Residues: 1-305; Coverage: 100%)
Gene name: MM_2353
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2221
Unit cell:
a: 117.144Å b: 182.5Å c: 152.046Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.217 0.241
Expression system: Escherichia coli BL21(DE3)