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Dcsbis, a diguanylate cyclase from Pseudomonas aeruginosa

X-ray diffraction
2.502Å resolution
PDB entry 4zmu coloured by chain, front view.
PDB entry 4zmu coloured by chain, side view.
PDB entry 4zmu coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: diguanylate cyclase
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Multimeric state: homo dimer
Accessible surface area: 27623.94 Å2
Buried surface area: 3054.44 Å2
Dissociation area: 1,527.22 Å2
Dissociation energy (ΔGdiss): 15.1 kcal/mol
Dissociation entropy (TΔSdiss): 14.08 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-191224
Assembly 2
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Assembly Name: diguanylate cyclase
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Multimeric state: homo dimer
Accessible surface area: 27325.45 Å2
Buried surface area: 3165.8 Å2
Dissociation area: 1,582.9 Å2
Dissociation energy (ΔGdiss): 14.04 kcal/mol
Dissociation entropy (TΔSdiss): 14.08 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-191224

Macromolecules

diguanylate cyclase
Chains: A, B, C, D
Length: 345 amino acids
Theoretical weight: 38.38 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I072 (Residues: 1-341; Coverage: 100%)
Gene name: PA2771
Pfam:
InterPro:
CATH: Alpha-Beta Plaits
diguanylate cyclase in PDB entry 4zmu, assembly 1, front view.
diguanylate cyclase in PDB entry 4zmu, assembly 1, side view.
diguanylate cyclase in PDB entry 4zmu, assembly 1, top view.
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