4zmu

X-ray diffraction
2.5Å resolution

Dcsbis, a diguanylate cyclase from Pseudomonas aeruginosa

Released:
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Chen Y, Liu C, Liu S, Chi K, Gu L

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GGDEF domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 345 amino acids
Theoretical weight: 38.38 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I072 (Residues: 1-341; Coverage: 100%)
Gene name: PA2771
Sequence domains:
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 73.609Å b: 109.083Å c: 111.85Å
α: 90° β: 95.88° γ: 90°
R-values:
R R work R free
0.19 0.188 0.246
Expression system: Escherichia coli BL21(DE3)