› 4zl9

X-ray diffraction
1.7Å resolution

Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal III

Released:
DOI: 10.2210/pdb4zl9/pdb
PDB entry 4zl9 coloured by chain, front view.
PDB entry 4zl9 coloured by chain, side view.
PDB entry 4zl9 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Sent packing: protein engineering generates a new crystal form of Pseudomonas aeruginosa DsbA1 with increased catalytic surface accessibility.
McMahon RM, Coinçon M, Tay S, Heras B, Morton CJ, Scanlon MJ, Martin JL
Acta Crystallogr D Biol Crystallogr 71 2386-95 (2015)
PMID: 26627647

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142993 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiol:disulfide interchange protein DsbA Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 21.35 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0C2B2 (Residues: 22-211; Coverage: 100%)
Gene names: PA5489, dsbA
Sequence domains: DSBA-like thioredoxin domain
Structure domains: Glutaredoxin

Ligands and Environments

3 bound ligands:
Ligand MES 1 x MES
Ligand EDO 1 x EDO
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 35.39Å b: 62.86Å c: 42.09Å
α: 90° β: 98.23° γ: 90°
R-values:
R R work R free
0.161 0.159 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Fragment library screening identifies hits that bind to the non-catalytic surface of Pseudomonas aeruginosa DsbA1.
Mohanty et al. (2017)
2 more

1 mention without citation

Sent packing: protein engineering generates a new crystal form of Pseudomonas aeruginosa DsbA1 with increased catalytic surface accessibility.
McMahon et al. (2015)