PDBe 4zks

X-ray diffraction
1.85Å resolution

The crystal structure of upain-1-W3A in complex with inactive uPA (uPA-S195A) at pH7.4

Released:
Entry authors: Jiang L, Andreasen PA, Huang M

Function and Biology Details

Reaction catalysed:
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Urokinase-type plasminogen activator chain B Chain: U
Molecule details ›
Chain: U
Length: 247 amino acids
Theoretical weight: 27.83 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00749 (Residues: 179-425; Coverage: 60%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
upain-1-W3A Chain: P
Molecule details ›
Chain: P
Length: 12 amino acids
Theoretical weight: 1.23 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R3
Unit cell:
a: 120.751Å b: 120.751Å c: 42.961Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.245
Expression systems:
  • Komagataella pastoris
  • Not provided