› 4zke

X-ray diffraction
2.25Å resolution

Crystal structure of the S. cerevisiae Ski7 GTPase-like domain, bound to GTP.

Released:
DOI: 10.2210/pdb4zke/pdb
PDB 4zke coloured by chain and viewed from the front.
PDB 4zke coloured by chain and viewed from the side.
PDB 4zke coloured by chain and viewed from the top.
Source organism: Saccharomyces cerevisiae
Primary publication:
Saccharomyces cerevisiae Ski7 Is a GTP-Binding Protein Adopting the Characteristic Conformation of Active Translational GTPases.
Kowalinski E, Schuller A, Green R, Conti E
Structure (2015)
PMID: 26051716

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superkiller protein 7 Chain: A
Molecule details ›
Chain: A
Length: 499 amino acids
Theoretical weight: 56.66 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08491 (Residues: 254-747; Coverage: 66%)
Gene names: SKI7, YOR076C, YOR29-27
Sequence domains: Elongation factor Tu GTP binding domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand GTP 1 x GTP
Ligand NA 1 x NA
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 91.15Å b: 123.106Å c: 104.967Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.205 0.239
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Catalytic activities, molecular connections, and biological functions of plant RNA exosome complexes.
Lange et al. (2022)
6 more

3 mentions without citation

Targeting RNA for processing or destruction by the eukaryotic RNA exosome and its cofactors.
Zinder et al. (2017)
2 more