› 4zin

X-ray diffraction
1.67Å resolution

Genetically encoded Phenyl Azide Photochemistry Drive Positive and Negative Functional Modulation of a Red Fluorescent Protein

Released:
DOI: 10.2210/pdb4zin/pdb
PDB 4zin coloured by chain and viewed from the front.
PDB 4zin coloured by chain and viewed from the side.
PDB 4zin coloured by chain and viewed from the top.
Source organism: Anaplasma marginale
Primary publication:
Genetically encoded phenyl azide photochemistry drives positive and negative functional modulation of a red fluorescent protein
Reddington SC, Driezis S, Hartley AM, Watson PD, Rizkallah PJ, Jones DD
RSC Adv 5 77734-77738 (2015)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MCherry fluorescent protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 217 amino acids
Theoretical weight: 24.78 KDa
Source organism: Anaplasma marginale
Expression system: Escherichia coli
UniProt:
  • Canonical: X5DSL3 (Residues: 11-229; Coverage: 93%)
Gene name: mCherry
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand EDO 1 x EDO
1 modified residue:
Ligand CH6 3 x CH6

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 43.28Å b: 103.43Å c: 150.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.248
Expression system: Escherichia coli

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