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X-ray diffraction
2.01Å resolution

Phototoxic Fluorescent Protein KillerOrange

Released:
DOI: 10.2210/pdb4zfs/pdb
PDB 4zfs coloured by chain and viewed from the front.
PDB 4zfs coloured by chain and viewed from the side.
PDB 4zfs coloured by chain and viewed from the top.
Source organism: Hydrozoa
Primary publication:
Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore.
Pletneva NV, Pletnev VZ, Sarkisyan KS, Gorbachev DA, Egorov ES, Mishin AS, Lukyanov KA, Dauter Z, Pletnev S
PLoS ONE 10 e0145740 (2015)
PMID: 26699366

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
KillerRed Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 246 amino acids
Theoretical weight: 27.63 KDa
Source organism: Hydrozoa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2TCH5 (Residues: 2-237; Coverage: 99%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 128.93Å b: 202.064Å c: 116.679Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.236
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Advances in the Genetically Engineered KillerRed for Photodynamic Therapy Applications.
Liu et al. (2021)
5 more

1 mention without citation

Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore.
Pletneva et al. (2015)