› 4zfq

X-ray diffraction
2.8Å resolution

Structure of M. tuberculosis (3,3) L,D-Transpeptidase, LdtMt5. (Meropenen-adduct form)

Released:
DOI: 10.2210/pdb4zfq/pdb
PDB 4zfq coloured by chain and viewed from the front.
PDB 4zfq coloured by chain and viewed from the side.
PDB 4zfq coloured by chain and viewed from the top.
Source organism: Mycobacterium tuberculosis CDC1551
Primary publication:
Loss of a functionally and structurally distinct L,D-transpeptidase, LdtMt5, compromises cell wall integrity in Mycobacterium tuberculosis.
Brammer Basta LA, Ghosh A, Pan Y, Jakoncic J, Lloyd EP, Townsend CA, Lamichhane G, Bianchet MA
J. Biol. Chem. (2015)
PMID: 26304120

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L,D-transpeptidase 5 Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 47.93 KDa
Source organism: Mycobacterium tuberculosis CDC1551
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WKV2 (Residues: 1-451; Coverage: 100%)
Gene name: MT0501
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PEG 2 x PEG
Ligand DWZ 1 x DWZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P6222
Unit cell:
a: 99.393Å b: 99.393Å c: 193.384Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.23 0.277
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Building peptidoglycan inside eukaryotic cells: A view from symbiotic and pathogenic bacteria.
García-Del Portillo F. (2020)
4 more

2 mentions without citation

Loss of a Functionally and Structurally Distinct ld-Transpeptidase, LdtMt5, Compromises Cell Wall Integrity in Mycobacterium tuberculosis.
Brammer Basta et al. (2015)
1 more