X-ray diffraction
1.82Å resolution

Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(Cl1Y), H148D; circular permutant (50-51)

Source organism: Aequorea victoria

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.23 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P42212 (Residues: 51-237; Coverage: 79%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 51.84Å b: 68.59Å c: 60.88Å
α: 90° β: 100.5° γ: 90°
R R work R free
0.201 0.198 0.246
Expression system: Escherichia coli BL21(DE3)