› 4zf4

X-ray diffraction
1.82Å resolution

Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(Cl1Y), H148D; circular permutant (50-51)

Released:
DOI: 10.2210/pdb4zf4/pdb
PDB 4zf4 coloured by chain and viewed from the front.
PDB 4zf4 coloured by chain and viewed from the side.
PDB 4zf4 coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Primary publication:
Short Hydrogen Bonds and Proton Delocalization in Green Fluorescent Protein (GFP).
Oltrogge LM, Boxer SG
ACS Cent Sci 1 148-56 (2015)
PMID: 27162964

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.23 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 51-237; Coverage: 79%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 4NT 2 x 4NT

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 51.84Å b: 68.59Å c: 60.88Å
α: 90° β: 100.5° γ: 90°
R-values:
R R work R free
0.201 0.198 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Low barrier hydrogen bonds in protein structure and function.
Kemp et al. (2021)
1 more

2 mentions without citation

Short Hydrogen Bonds and Proton Delocalization in Green Fluorescent Protein (GFP).
Oltrogge et al. (2015)
1 more