› 4zbp

X-ray diffraction
2.6Å resolution

Crystal structure of the AMPCPR-bound AtNUDT7

Released:
DOI: 10.2210/pdb4zbp/pdb
PDB 4zbp coloured by chain and viewed from the front.
PDB 4zbp coloured by chain and viewed from the side.
PDB 4zbp coloured by chain and viewed from the top.
Source organism: Arabidopsis thaliana
Primary publication:
Crystal Structures of Arabidopsis thaliana Nudix Hydrolase NUDT7 Reveal a Previously Unobserved Conformation.
Tang Q, Liu C, Zhong C, Ding J
Mol Plant (2015)
PMID: 26253775

Function and Biology Details

Reactions catalysed: 
NAD(H) + H(2)O = AMP + NMN(H)
ADP-D-ribose + H(2)O = AMP + D-ribose 5-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nudix hydrolase 7 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 310 amino acids
Theoretical weight: 35.19 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SU14 (Residues: 1-282; Coverage: 100%)
Gene names: At4g12720, GFG1, NUDT7, NUDX7, T20K18.70
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand ADV 1 x ADV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2221
Unit cell:
a: 48.202Å b: 258.72Å c: 157.548Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.238 0.29
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Structural basis of prokaryotic NAD-RNA decapping by NudC.
Zhang et al. (2016)