› 4zbo

X-ray diffraction
1.4Å resolution

Streptomyces bingchenggensis acetoacetate decarboxylase in non-covalent complex with potassium formate

Released:
DOI: 10.2210/pdb4zbo/pdb
PDB entry 4zbo coloured by chain, front view.
PDB entry 4zbo coloured by chain, side view.
PDB entry 4zbo coloured by chain, top view.
Source organism: Streptomyces bingchenggensis BCW-1
Primary publication:
Sbi00515, a Protein of Unknown Function from Streptomyces bingchenggensis, Highlights the Functional Versatility of the Acetoacetate Decarboxylase Scaffold.
Mydy LS, Hoppe RW, Ochsenwald JM, Berndt RT, Severin GB, Schwabacher AW, Silvaggi NR
Biochemistry 54 3978-88 (2015)
PMID: 26039798

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetoacetate decarboxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 265 amino acids
Theoretical weight: 28.17 KDa
Source organism: Streptomyces bingchenggensis BCW-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D7C0E5 (Residues: 1-265; Coverage: 100%)
Gene name: SBI_00515
Sequence domains: Acetoacetate decarboxylase (ADC)
Structure domains: Acetoacetate decarboxylase-like

Ligands and Environments

4 bound ligands:
Ligand FMT 11 x FMT
Ligand PEG 5 x PEG
Ligand PE4 1 x PE4
Ligand ETE 1 x ETE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21212
Unit cell:
a: 157.9Å b: 124.4Å c: 53.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.155 0.175
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 mentions without citation

Self-association of a highly charged arginine-rich cell-penetrating peptide.
Tesei et al. (2017)
1 more