› 4za1

X-ray diffraction
2.5Å resolution

Crystal Structure of NosA Involved in Nosiheptide Biosynthesis

Released:
DOI: 10.2210/pdb4za1/pdb
PDB entry 4za1 coloured by chain, front view.
PDB entry 4za1 coloured by chain, side view.
PDB entry 4za1 coloured by chain, top view.
Source organism: Streptomyces actuosus
Primary publication:
Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis.
Liu S, Guo H, Zhang T, Han L, Yao P, Zhang Y, Rong N, Yu Y, Lan W, Wang C, Ding J, Wang R, Liu W, Cao C
Sci Rep 5 12744 (2015)
PMID: 26244829

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NosA Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 151 amino acids
Theoretical weight: 16.3 KDa
Source organism: Streptomyces actuosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C6FX40 (Residues: 1-151; Coverage: 100%)
Gene name: nosA
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P4132
Unit cell:
a: 143.371Å b: 143.371Å c: 143.371Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.263 0.179 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

YcaO-Dependent Posttranslational Amide Activation: Biosynthesis, Structure, and Function.
Burkhart et al. (2017)
2 more

3 mentions without citation

New developments in RiPP discovery, enzymology and engineering.
Montalbán-López et al. (2021)
2 more