› 4z7x

X-ray diffraction
1.55Å resolution

MdbA protein, a thiol-disulfide oxidoreductase from Actinomyces oris.

Released:
DOI: 10.2210/pdb4z7x/pdb
PDB entry 4z7x coloured by chain, front view.
PDB entry 4z7x coloured by chain, side view.
PDB entry 4z7x coloured by chain, top view.
Source organism: Actinomyces oris
Primary publication:
A Disulfide Bond-forming Machine Is Linked to the Sortase-mediated Pilus Assembly Pathway in the Gram-positive Bacterium Actinomyces oris.
Reardon-Robinson ME, Osipiuk J, Chang C, Wu C, Jooya N, Joachimiak A, Das A, Ton-That H
J Biol Chem 290 21393-405 (2015)
PMID: 26170452

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102467 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin-like fold domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 25.61 KDa
Source organism: Actinomyces oris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0M3KL32 (Residues: 1-238; Coverage: 100%)
Gene names: ANA_1994, mdbA
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand 3CX 2 x 3CX
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 46.88Å b: 66.86Å c: 256.169Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.127 0.124 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Disulfide-Bond-Forming Pathways in Gram-Positive Bacteria.
Reardon-Robinson et al. (2015)
6 more

3 mentions without citation

A Disulfide Bond-forming Machine Is Linked to the Sortase-mediated Pilus Assembly Pathway in the Gram-positive Bacterium Actinomyces oris.
Reardon-Robinson et al. (2015)
2 more