4z6x

X-ray diffraction
1.68Å resolution

The 1.68-angstrom crystal structure of acitive-site metal-free PqqB from Pseudomonas putida

Released:
Source organism: Pseudomonas putida KT2440
Entry authors: Tu X, Wilmot CM

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coenzyme PQQ synthesis protein B Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 34.12 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q88QV5 (Residues: 1-303; Coverage: 100%)
Gene names: PP_0379, pqqB
Sequence domains: Beta-lactamase superfamily domain
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P43212
Unit cell:
a: 86.694Å b: 86.694Å c: 106.871Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.137 0.175
Expression system: Escherichia coli BL21(DE3)