Structure analysis

Structure of the enzyme-product complex resulting from TDG action on a GU mismatch in the presence of excess base

X-ray diffraction
1.45Å resolution
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Multimeric state: hetero trimer
Accessible surface area: 18000 Å2
Buried surface area: 5600 Å2
Dissociation area: 2,400 Å2
Dissociation energy (ΔGdiss): 41 kcal/mol
Dissociation entropy (TΔSdiss): 24 kcal/mol
Interface energy (ΔGint): -15 kcal/mol
Symmetry number: 1

Macromolecules

Chain: A
Length: 204 amino acids
Theoretical weight: 23.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13569 (Residues: 111-308; Coverage: 48%)
Gene name: TDG
Pfam: Uracil DNA glycosylase superfamily
InterPro:
CATH: Uracil-DNA glycosylase-like domain

Search similar proteins

Name: DNA
Representative chains: C
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 28 nucleotides
Theoretical weight: 8.65 KDa

Search similar DNA

Name: DNA
Representative chains: D
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 28 nucleotides
Theoretical weight: 8.47 KDa

Search similar DNA