X-ray diffraction
2.45Å resolution

Crystal Structure of 2-hydroxybiphenyl 3-monooxygenase W225Y from Pseudomonas azelaica


Function and Biology Details

Reaction catalysed:
2-hydroxybiphenyl + NADH + O(2) = 2,3-dihydroxybiphenyl + NAD(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
FAD_binding_3 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 592 amino acids
Theoretical weight: 64.64 KDa
Source organism: Pseudomonas nitroreducens HBP1
Expression system: Escherichia coli
  • Canonical: O06647 (Residues: 2-586; Coverage: 100%)
Gene name: hbpA
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments

Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 154.38Å b: 130.58Å c: 78.75Å
α: 90° β: 98.79° γ: 90°
R R work R free
0.219 0.218 0.236
Expression system: Escherichia coli