4z2c

X-ray diffraction
3.19Å resolution

Quinolone(Moxifloxacin)-DNA cleavage complex of gyrase from S. pneumoniae

Released:
Source organism: Streptococcus pneumoniae
Entry authors: Laponogov I, Veselkov DA, Pan X-S, Selvarajah J, Crevel IM-T, Fisher LM, Sanderson MR

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
DNA gyrase subunit A Chains: A, B
Molecule details ›
Chains: A, B
Length: 499 amino acids
Theoretical weight: 56.75 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DPM2 (Residues: 1-493; Coverage: 60%)
Gene names: gyrA, spr1099
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
DNA gyrase subunit B Chains: C, D
Molecule details ›
Chains: C, D
Length: 269 amino acids
Theoretical weight: 30.29 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A4M0 (Residues: 404-648; Coverage: 38%)
Gene names: gyrB, spr0715
Sequence domains:
Symmetrized E-site DNA Chains: E, G
Molecule details ›
Chains: E, G
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Symmetrized E-site DNA Chains: F, H
Molecule details ›
Chains: F, H
Length: 19 nucleotides
Theoretical weight: 5.82 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 92.03Å b: 94.95Å c: 274.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.268
Expression system: Escherichia coli