› 4z0c

X-ray diffraction
2.3Å resolution

Crystal structure of TLR13-ssRNA13 complex

Released:
DOI: 10.2210/pdb4z0c/pdb
PDB 4z0c coloured by chain and viewed from the front.
PDB 4z0c coloured by chain and viewed from the side.
PDB 4z0c coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Structural basis for specific recognition of single-stranded RNA by Toll-like receptor 13.
Song W, Wang J, Han Z, Zhang Y, Zhang H, Wang W, Chang J, Xia B, Fan S, Zhang D, Wang J, Wang HW, Chai J
Nat. Struct. Mol. Biol. (2015)
PMID: 26323037

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
Toll-like receptor 13 Chains: A, D
Molecule details ›
Chains: A, D
Length: 709 amino acids
Theoretical weight: 81.34 KDa
Source organism: Mus musculus
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q6R5N8 (Residues: 69-777; Coverage: 77%)
Gene name: Tlr13
Sequence domains:
DNA (5'-R(P*AP*CP*GP*GP*AP*AP*AP*GP*AP*CP*CP*CP*C)-3') Chains: B, C
Molecule details ›
Chains: B, C
Length: 13 nucleotides
Theoretical weight: 4.16 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 20 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 112.549Å b: 115.209Å c: 167.611Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.244
Expression systems:
  • Insect cell expression vector pTIE1
  • Not provided

Quick links

Citations

12 review citations

Regulation of the nucleic acid-sensing Toll-like receptors.
Lind et al. (2022)
11 more

6 mentions without citation

Host-pathogen protein-nucleic acid interactions: A comprehensive review.
Jain et al. (2022)
5 more