X-ray diffraction
2.38Å resolution

Crystal Structure of Streptococcus pneumoniae NanC, in complex with Oseltamivir.

Entry authors: Lukacik P, Owen CD, Potter JA, Taylor GL, Walsh MA

Function and Biology Details

Reaction catalysed:
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Sialidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 677 amino acids
Theoretical weight: 76 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A0A0H2UQE4 (Residues: 84-740; Coverage: 92%)
Gene name: SP_1326
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 73.51Å b: 136.03Å c: 150.17Å
α: 90° β: 90° γ: 90°
R R work R free
0.218 0.216 0.267
Expression system: Escherichia coli BL21(DE3)