› 4yz2

X-ray diffraction
2.06Å resolution

Crystal Structure of Streptococcus pneumoniae NanC, in complex with 2-deoxy-2,3-didehydro-N-acetylneuraminic acid.

Released:
DOI: 10.2210/pdb4yz2/pdb
PDB entry 4yz2 coloured by chain, front view.
PDB entry 4yz2 coloured by chain, side view.
PDB entry 4yz2 coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
Streptococcus pneumoniae NanC: STRUCTURAL INSIGHTS INTO THE SPECIFICITY AND MECHANISM OF A SIALIDASE THAT PRODUCES A SIALIDASE INHIBITOR.
Owen CD, Lukacik P, Potter JA, Sleator O, Taylor GL, Walsh MA
J Biol Chem 290 27736-48 (2015)
PMID: 26370075

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101451 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative neuraminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 677 amino acids
Theoretical weight: 76 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2UQE4 (Residues: 84-740; Coverage: 92%)
Gene name: SP_1326
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand DAN 2 x DAN
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 73.3Å b: 136.71Å c: 150.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Glycan-metabolizing enzymes in microbe-host interactions: the Streptococcus pneumoniae paradigm.
Hobbs et al. (2018)
2 more

2 mentions without citation

Streptococcus pneumoniae NanC: STRUCTURAL INSIGHTS INTO THE SPECIFICITY AND MECHANISM OF A SIALIDASE THAT PRODUCES A SIALIDASE INHIBITOR.
Owen et al. (2015)
1 more