› 4yxh

X-ray diffraction
2.7Å resolution

Crystal structure of Deer prion protein complexed with POM1 FAB

Released:
DOI: 10.2210/pdb4yxh/pdb
PDB 4yxh coloured by chain and viewed from the front.
PDB 4yxh coloured by chain and viewed from the side.
PDB 4yxh coloured by chain and viewed from the top.
Source organisms:
Primary publication:
X-ray structural and molecular dynamical studies of the globular domains of cow, deer, elk and Syrian hamster prion proteins.
Baral PK, Swayampakula M, Aguzzi A, James MN
J. Struct. Biol. (2015)
PMID: 26320075

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Major prion protein Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.96 KDa
Source organism: Odocoileus hemionus
Expression system: Escherichia coli
UniProt:
  • Canonical: P47852 (Residues: 124-231; Coverage: 47%)
Gene names: PRNP, PRP
POM1 FAB HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 23.4 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
POM1 FAB LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 84.282Å b: 106.58Å c: 75.775Å
α: 90° β: 95.81° γ: 90°
R-values:
R R work R free
0.245 0.242 0.288
Expression systems:
  • Escherichia coli
  • Mus musculus

Quick links

Citations

1 review citation

Conformational selection in amyloid-based immunotherapy: Survey of crystal structures of antibody-amyloid complexes.
Ma et al. (2016)
4 other articles

2 mentions without citation

In vitro generation of tau aggregates conformationally distinct from parent tau seeds of Alzheimer's brain.
Nam et al. (2019)
1 more