› 4yx2

X-ray diffraction
2.19Å resolution

Crystal structure of Bovine prion protein complexed with POM1 FAB

Released:
DOI: 10.2210/pdb4yx2/pdb
PDB 4yx2 coloured by chain and viewed from the front.
PDB 4yx2 coloured by chain and viewed from the side.
PDB 4yx2 coloured by chain and viewed from the top.
Source organisms:
Primary publication:
X-ray structural and molecular dynamical studies of the globular domains of cow, deer, elk and Syrian hamster prion proteins.
Baral PK, Swayampakula M, Aguzzi A, James MN
J. Struct. Biol. (2015)
PMID: 26320075

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Major prion protein Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.49 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10279 (Residues: 103-242; Coverage: 58%)
Gene names: PRNP, PRP
POM1 FAB HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 218 amino acids
Theoretical weight: 23.4 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
POM1 FAB LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: C2
Unit cell:
a: 89.674Å b: 99.764Å c: 75.942Å
α: 90° β: 93.08° γ: 90°
R-values:
R R work R free
0.218 0.216 0.263
Expression systems:
  • Escherichia coli
  • Mus musculus

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Citations

1 review citation

Conformational selection in amyloid-based immunotherapy: Survey of crystal structures of antibody-amyloid complexes.
Ma et al. (2016)
7 other articles