› 4ywc

X-ray diffraction
2.4Å resolution

Crystal structure of Myc3(5-242) fragment in complex with Jaz9(218-239) peptide

Released:
DOI: 10.2210/pdb4ywc/pdb
PDB entry 4ywc coloured by chain, front view.
PDB entry 4ywc coloured by chain, side view.
PDB entry 4ywc coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling.
Zhang F, Yao J, Ke J, Zhang L, Lam VQ, Xin XF, Zhou XE, Chen J, Brunzelle J, Griffin PR, Zhou M, Xu HE, Melcher K, He SY
OpenAccess logo Nature 525 269-73 (2015)
PMID: 26258305

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186663 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription factor MYC3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 25.95 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9FIP9 (Residues: 5-242; Coverage: 40%)
Gene names: ATR2, At5g46760, BHLH5, EN36, MYC3, MZA15.18
Sequence domains: bHLH-MYC and R2R3-MYB transcription factors N-terminal
Protein TIFY 7 Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 amino acids
Theoretical weight: 2.62 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
UniProt:
  • Canonical: Q8W4J8 (Residues: 218-239; Coverage: 8%)
Gene names: At1g70700, F5A18.12, JAZ9, TIFY7
Sequence domains: Jas motif

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 59.105Å b: 110.627Å c: 161.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.239 0.295
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

32 review citations

Jasmonates: biosynthesis, metabolism, and signaling by proteins activating and repressing transcription.
Wasternack et al. (2017)
31 more

3 mentions without citation

Intrinsic Disorder in Plant Transcription Factor Systems: Functional Implications.
Salladini et al. (2020)
2 more