› 4yvb

X-ray diffraction
1.35Å resolution

Structure of D128N streptavidin

Released:
DOI: 10.2210/pdb4yvb/pdb
PDB 4yvb coloured by chain and viewed from the front.
PDB 4yvb coloured by chain and viewed from the side.
PDB 4yvb coloured by chain and viewed from the top.
Source organism: Streptomyces avidinii
Entry authors: Baugh L, Le Trong I, Stayton PS, Stenkamp RE, Lybrand TP

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 120 amino acids
Theoretical weight: 12.69 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 40-159; Coverage: 76%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand BTN 4 x BTN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 50.597Å b: 98.95Å c: 52.638Å
α: 90° β: 112.58° γ: 90°
R-values:
R R work R free
0.129 0.127 0.17
Expression system: Escherichia coli

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