› 4yus

X-ray diffraction
1.8Å resolution

Crystal structure of photoactivated adenylyl cyclase of a cyanobacteriaOscillatoria acuminata in hexagonal form

Released:
DOI: 10.2210/pdb4yus/pdb
PDB entry 4yus coloured by chain, front view.
PDB entry 4yus coloured by chain, side view.
PDB entry 4yus coloured by chain, top view.
Source organism: Oscillatoria acuminata PCC 6304
Primary publication:
Structural insight into photoactivation of an adenylate cyclase from a photosynthetic cyanobacterium.
Ohki M, Sugiyama K, Kawai F, Tanaka H, Nihei Y, Unzai S, Takebe M, Matsunaga S, Adachi S, Shibayama N, Zhou Z, Koyama R, Ikegaya Y, Takahashi T, Tame JR, Iseki M, Park SY
Proc Natl Acad Sci U S A 113 6659-64 (2016)
PMID: 27247413

Function and Biology Details

Reaction catalysed: 
ATP = 3',5'-cyclic AMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125708 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Family 3 adenylate cyclase Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 43.05 KDa
Source organism: Oscillatoria acuminata PCC 6304
Expression system: Escherichia coli
UniProt:
  • Canonical: K9TLZ5 (Residues: 1-366; Coverage: 100%)
Gene name: Oscil6304_3613
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand FMN 1 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P6122
Unit cell:
a: 76.838Å b: 76.838Å c: 204.778Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.212 0.264
Expression system: Escherichia coli

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Citations

13 review citations

Blue-Light Receptors for Optogenetics.
Losi et al. (2018)
12 more

7 mentions without citation

Seeing the light with BLUF proteins.
Park et al. (2017)
6 more